Importance of Mutant Position in Ramachandran Plot for Predicting Protein Stability upon Surface Mutations
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چکیده
منابع مشابه
CUPSAT: Predicting Protein Stability Upon Point Mutations
CUPSAT is a web tool to analyse and predict protein stability changes upon point mutations (single amino acid mutations) in proteins. These mutations are carried out experimentally using site-directed mutagenesis and similar techniques. Random mutations at a specified position aid in designing thermostable or thermosensitive proteins so that the functionality of a protein can be altered to suit...
متن کاملPrediction of protein stability upon point mutations.
Prediction of protein stability upon amino acid substitution is a challenging problem and it will be helpful for designing stable mutants. We have developed a thermodynamic database for proteins and mutants (ProTherm), which has more than 20000 thermodynamic data along with sequence and structure information, experimental conditions and literature information. It is freely accessible at http://...
متن کاملRamachandran plot on the web
A graphics package has been developed to display the main chain torsion angles phi, psi (phi, Psi); (Ramachandran angles) in a protein of known structure. In addition, the package calculates the Ramachandran angles at the central residue in the stretch of three amino acids having specified the flanking residue types. The package displays the Ramachandran angles along with a detailed analysis ou...
متن کاملiPTREE-STAB: interpretable decision tree based method for predicting protein stability changes upon mutations
UNLABELLED We have developed a web server, iPTREE-STAB for discriminating the stability of proteins (stabilizing or destabilizing) and predicting their stability changes (delta deltaG) upon single amino acid substitutions from amino acid sequence. The discrimination and prediction are mainly based on decision tree coupled with adaptive boosting algorithm, and classification and regression tree,...
متن کاملA neural-network-based method for predicting protein stability changes upon single point mutations
MOTIVATION One important requirement for protein design is to be able to predict changes of protein stability upon mutation. Different methods addressing this task have been described and their performance tested considering global linear correlation between predicted and experimental data. Neither is direct statistical evaluation of their prediction performance available, nor is a direct compa...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2000
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.40.s117_2